UWS Nepean had two campuses:  Penrith (sites at Kingswood, Werrington North and Werrington South) and Parramatta (sites at Parramatta and Westmead).

The Werrington site of Nepean's Kingswood/Werrington campus was part of the colonial government's land grant to Mary Putland, Captain Bligh's daughter.  The historic house known as Frogmore that still stands overlooking the Werrington site has been restored and accommodates UWS headquarters.

The Kingswood/Werrington campus is on both sides of the Great Western Highway between Penrith and St Marys.  The dual site has been linked since early 1991 by a steel and concrete span bridge crossing the highway.

The joint Werrington/Kingswood campus covers a total of 148 hectares and serves all six teaching Schools of Nepean:  Business, Education, Visual and Performing Arts, Science and Technology, Humanities and Applied Social Sciences, and Health Studies.

A major new campus at Parramatta on the corner of James Ruse Drive and Victoria Road was developed on the site of the old Rydalmere Institution (UWS Annual Report 1996, p.7).

The future of the UWS federation dominated 1995, following the resolution by Nepean's Academic Committee that it believed UWS Nepean would better achieve its Mission as a university in its own right.  Following an endorsement by the Board of Governors of the continuance of a single UWS, the senior staff within UWS Nepean accepted the decision and have participated in the process of restructuring the federated organisation of the University (UWS Annual Report 1995, pp.15-16).

As the result of a major restructure, UWS Nepean became part of the University of Western Sydney which, from the start of 2001, operated as a single multi-campus university rather than as a federation.